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Poster De Conférence Année : 2023

Intensification of oleosomes aqueous extraction from Camelina sativa seeds: towards a sustainable and minimally processed natural ingredient

Résumé

Food and bioproducts emulsion-based products are generally produced from refined oils that are emulsified while adding emulsifiers and stabilizers. An alternative processing approach is to extract the natively stable oil-storing structures, oleosomes, and to use them as a natural ingredient for further applications. This study aimed to develop a scalable minimal process for the recovery of oleosomes at high yield from Camelina sativa seeds and the preservation of their native structural and functional properties. Camelina sativa presents not only advantageous agronomical behavior but also seeds rich in W-3 fatty acids compartmentally located in oleosomes (30–38% of the seed mass) and in soluble fibers (7–10% of the seed mass). Like for many oleaginous seeds, the outermost layer of Camelina seeds are surrounded by a mucilage that expands under hydration. This results in a thickening effect that hinders shear forces during mechanical treatments and interferes with oleosomes recovery. The process developed in this study includes several cascade steps that were designed to cope with this behavior, while seeking for high oleosome recovery yield and optimized energy and water consumptions. It includes: i) Soaking (5% seeds w/w) in order to hydrate and break the mucilaginous network. ii) Sonication using a continuous system equipped with a cascatrode and operating at 670 W for 30 s, completed by a fluidic recirculation (350 L/h) as an efficient pre-treatment for mucilage removal by iii) washing in a drum separator (0.5 mm sieve mesh). iv) Demucilated seeds are then suspended in water (5 to 25% w/w) and grinded using a colloid mill set with a variable gap between rotor and stator devices and operated at 7990 rpm for 5 to 15 min. The final separation step is made by centrifugation. The operating parameters were combined in order to identify the best performing conditions in terms of oleosome recovery yield and quality. Samples were characterized by laser granulometry and confocal microscopy, proving the integrity of extracted structures. Energy consumption was evaluated throughout the processing steps. Water footprint minimization would be an interesting way to improve the environmental friendliness. The composition of mucilage suspension would be analyzed for further applications.
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Dates et versions

hal-04484861 , version 1 (29-02-2024)

Identifiants

  • HAL Id : hal-04484861 , version 1

Citer

Marwen Moussa, Pablo Granda, Delphine Huc-Mathis, Paola Soto, Sabine D’andréa, et al.. Intensification of oleosomes aqueous extraction from Camelina sativa seeds: towards a sustainable and minimally processed natural ingredient: Intensification of oleosomes aqueous extraction from Camelina sativa seeds: towards a sustainable and minimally processed natural ingredient. 7th International ISEKI-Food Conference, Jul 2023, Palaiseau, France. ⟨hal-04484861⟩
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