In-vitro production of standards to identify biomarkers of exposure and effect of the emerging mycotoxin Versicolorin A
Résumé
The toxicity of bisfuranoid mycotoxins, like aflatoxin B1 (AFB1) or sterigmatocystin, is mostly determined by their biotransformation, detoxification, or bioactivation. It is well established that the in -vivo biotransformation of AFB1 into a highly reactive molecule is the key step in the genotoxic process and therefore in carcinogenesis [1]. Hence, the presence of a bisfuran moiety in Versicolorin A (VerA), a precursor of AFB1 and co-detected with AFB1 in food, may also explain its genotoxicity. VerA is not commercially available and its metabolization has not been studied so far. Therefore, the purpose of this study was to establish the characterization of metabolites derived from synthetized VerA. To this aim, our strategy was based on the construction of a library of VerA metabolites using human in vitro S9 fraction incubations in the presence of appropriate cofactors coupled to UHPLC-ESI-HRMS [2]. The resulting chromatographic and mass-spectrometric data were used to identify VerA metabolites produced by intestinal cell lines as well as intestinal and liver tissues exposed ex vivo.
Domaines
Chimie analytique| Origine | Fichiers produits par l'(les) auteur(s) |
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